The effects of biopsy and cryopreservation by vitrification on the in vitro development of eight-cell mouse embryos
Document Type
Article
Publication Date
1-1-2006
Abstract
In vitro and in vivo development of cryopreserved and non-cryopreserved biopsied 8-cell mouse embryos were examined in this study. It was observed that intact and biopsied embryos could successfully be cryopreserved by using the blastomere biopsy and vitrification procedure. However, in vitro survivability of the embryos declined steadily with advancing developmental stages, irrespective of the treatments used. Overall, one-blastomere biopsy without cryopreservation yielded the highest rate of development to morula (96.1%), expanded blastocyst (84.3%) and hatched blastocyst (74.5%), respectively. When the effect of cryopreservation was taken into account, two-blastomere biopsy did not differ significantly from that of the one-blastomere biopsy in terms of development to the expanded and hatched blastocyst stages. The results of our study suggest that the two-blastomere biopsy with cryopreservation is a better option in the current set-up.
Keywords
Cryopreservation, Embryo biopsy, Mouse embryo, Preimplantation genetic diagnosis, article, biopsy, blastocyst, blastomere, developmental stage, embryo, embryo culture, in vitro study, morula, mouse, nonhuman, survival rate, vitrification
Divisions
InstituteofBiologicalSciences
Publication Title
Biomedical Research
Volume
17
Issue
1
Additional Information
Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA