Gender identification of mouse embryos using the polymerase chain reaction (PCR) technique
Document Type
Article
Publication Date
1-1-2006
Abstract
A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three bands, of sizes 217 bp (Zfy), 147 bp (Sry) and 111 bp (Nds). In contrast, amplification of female DNA gave a single band of 111 bp (Nds). Test on blood DNA showed a 98.0% PCR amplification efficiency whereas single blastomeres produced 93.0% PCR amplification efficiency. In conclusion, mouse embryos were successfully sexed within 6 hours by using single blastomeres obtained from 8-cell embryos.
Keywords
Embryo biopsy, Embryo sexing, Gender identification, DNA, Amplicon, Animal cell, Animal experiment, Article, Blastomere, Chromosome analysis, Controlled study, Dna determination, Dna polymorphism, Embryo, Female, Gender identity, Gene amplification, Genetic analysis, Male, Mouse, Nonhuman, Polymerase chain reaction, Sequence analysis, Sex determination, SRY gene, X chromosome, Y chromosome
Divisions
InstituteofBiologicalSciences
Publication Title
Biomedical Research
Volume
17
Issue
3
Additional Information
Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA