Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique
Document Type
Article
Publication Date
1-1-2006
Abstract
The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin (TRITC-LCA). The results showed that mean percentages of acrosome-intact sperm incubated in vitro were considerably low. After one-hour incubation there were 43.20±2.83, 31.81±8.44 and 30.90±4.15% for ICR, C57/6J and F1 sperm, respectively. Intact acrosome in ICR sperm was significantly reduced (P<0.05) to 16.95±4.23% after two-hour incubation, however, insignificant reduction (P>0.05) was found for C57/6J and F1 sperm, which were 30.03±2.06 and 20.93±3.81%, respectively. The results showed that both dyes FITC-PSA and TRITC-LCA suitably stained the mouse sperm and produced insignificant difference (P>0.05) in the percentages of acrosome-intact sperm, which were 40.03±4.20 and 49.79±4.63%, respectively. One-hour incubation was adequate to capacitate sperm in vitro. Low acrosome intact due to extended period of incubation and morphologically abnormal sperm could compromise in vitro fertilization rates.
Keywords
Sperm, Acrosome, Lectin, Pisum sativum agglutinin, Insemination
Divisions
InstituteofBiologicalSciences
Publication Title
Malaysian Applied Biology
Volume
35
Issue
1
Additional Information
Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA