Date of Award

2010

Thesis Type

Masters

Document Type

Dissertation

Divisions

Faculty of Science

Department

Institute of Biological Sciences

Institution

Universiti Malaya

Abstract

Ligninolytic enzymes of white-rot fungi have broad specificities and have been implicated in transformation and mineralization of organo-pollutant with chemical structure similar to lignin e.g. synthetic dyes. This study was carried out to optimize selected parameters for decolorization of Crystal violet by Pycnoporus sanguineus in shake flasks. The parameters studied were initial dye concentration (ppm), agitation speed (rpm) and process time. They were optimized using response surface methodology (RSM). Subsequent to the optimization, the decolorization process was scaled up in stirred tank reactor (STR). Effect of different geometry of impellers on the decolorization process was studied. Curved blade and angled blade 60° impellers were used in the STR. Statistical analysis showed that initial dye concentration and decolorization time have significant effect exerted on the dye decolorization process (p < 0.05). Optimum condition for crystal violet decolorization was 40 ppm of initial dye concentration, two days process time and 160 rpm of agitation speed. In the STR, when curved blade impeller was used, increasing its rotational speed will increase the percentage of decolorization. On the contrary, when angled blade 60° impeller was used, the percentage of decolorization decreases with the increase in rotational speed of the impeller.

Initial

khm

Additional Information

Dissertation (M.A.) – Faculty of Science, Universiti Malaya, 2010.

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