Habsah Aziz

Date of Award

1-1-2010

Thesis Type

masters

Document Type

Thesis

Divisions

science

Department

Institute of Biological Sciences

Institution

University of Malaya

Abstract

Peripheral blood stem cells (PBSC) have been used for transplantation since the early 1990s. CD34+ has been used as a marker of hematopoietic stem cells to determine the optimal timing for PBSC harvesting. However, a wide ranges of CD34+ cells cut-off values from 8/ul to 66/ul have been used to initiate PBSC harvesting in autologous patients. We performed here a study to determine the peripheral blood CD34+ cells cut-off value for the prediction of stem cell yield of 2 x106 CD34+ cells/kg body weight patients, to study the correlation between peripheral blood CD34+ cells and stem cell yield, to study the correlation between the day 1 post-apheresis and day 2 pre-apheresis CD34+ count and to study the collection efficiency of the apheresis protocol used. Forty eight patients with a total of 94 PBSC harvesting procedures were analyzed. All apheresis were performed using a COBE Spectra TM Apheresis System. Pre and post apheresis peripheral blood CD34+ cells as well as the harvested CD34+ cell count were analysed using FACSCalibur flow cytometer. The CD34+ harvesting cut-off value was determined using the Receiver Operating Characteristic (ROC) curve analysis and the correlation study was done using Pearson’s Correlation Analysis. The cut-off value of  27 cells/ul has been shown to predict a stem cell yield of  2 x106 CD34+ cells/ kg body weight with a single apheresis with 95% sensitivity and 82.4% specificity. Pre-apheresis peripheral blood CD34+ cell count correlated well with the stem cell yields (r = 0.963 and p<0.001). The day 1 post-apheresis peripheral blood CD34+ cell count correlates well with day 2 pre-apheresis count (r = 0.895), and a value of ≥ 25 cells/ul and may be able to predict the day 2 stem cell yield of  2 x106 CD34+ cells/ kg body weight. The median overall collection efficiency of the stem cell harvesting protocol was 63.7%. In conclusion, this study has shown that PB CD34+ count of  27 cells/ul will predict the stem cell yields of  2 x106 CD34+ cells/ kg body weight, and the apheresis protocol used for peripheral blood stem cell harvesting has gave satisfactory collection efficiency.

Note

Dissertation submitted in fulfillment of the requirement for the degree of Master of Biotechnology

Additional Files
3570-Habsah_SGF40020_Prelims.pdf (203 kB)
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