In vitro inhibition of melanin formation and enhancement of collagen production by a mushroom sclerotial water extract from the tiger milk mushroom, lignosus rhinocerus (agaricomycetes), with no skin and eye irritation

Document Type

Article

Publication Date

1-1-2022

Abstract

Lignosus rhinocerus has bioactive properties, such as anti-inflammatory, antioxidant, antiglycation, and immunomodulatory effects, that are beneficial to the skin. Gas chromatography-mass spectrometry analysis revealed the presence of cosmeceutical compounds, including linoleates, ascorbic acid dipahnitate, and all-trans-squalene, in the mushroom sclerotium. We further investigated the depigmentation and antiaging activities of L. rhinoceros cultivar TM02 sclerotia cold water extract (TM-CWE). TM-C WE at 10% (v/v) inhibited melanin production at the intro- and extracellular levels of B16-F1 melanoma cells (50.6-53.3%) and downregulated microplahalinia-associated transcription factor, the key protein for melanogenic enzyme expression, at the intracellular level (44.5 +/- 2.6%). The extract also promoted human procollagen type 1 production in HaCat cells by 51.30 +/- 2.87% at 12.5% (v/v). Irritation tests using three-dimensional eye and skin models further indicated that TM-CWE is a nonirritant with > 75% viability. Therefore, L. rhinoceros TM02 demonstrated the capacity to be a beneficial ingredient for skin care application.

Keywords

Lignosus rhinoceros, Tiger milk mushroom, Pigmentation, Human procollagen type 1, Nonirritant, Melanin, Cosmeceutical compounds, Medicinal mushrooms

Publication Title

International Journal of Medicinal Mushrooms

Divisions

fac_med

Funders

Fundamental Research Grant FRGS [Grant No: 2019-1 (FRGS/1/2019/SKK08/MAHSA/03/1)],SIRIM Fraunhofer Programme - Enhancement of Competitiveness and Market Access of SMEs (ECMAS) under the SIIMF Fund [Grant No: BIO 238/5/1]

Volume

24

Issue

5

Publisher

Begell House Inc

Publisher Location

50 NORTH ST, DANBURY, CT 06810 USA

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