In vitro inhibition of melanin formation and enhancement of collagen production by a mushroom sclerotial water extract from the tiger milk mushroom, lignosus rhinocerus (agaricomycetes), with no skin and eye irritation
Document Type
Article
Publication Date
1-1-2022
Abstract
Lignosus rhinocerus has bioactive properties, such as anti-inflammatory, antioxidant, antiglycation, and immunomodulatory effects, that are beneficial to the skin. Gas chromatography-mass spectrometry analysis revealed the presence of cosmeceutical compounds, including linoleates, ascorbic acid dipahnitate, and all-trans-squalene, in the mushroom sclerotium. We further investigated the depigmentation and antiaging activities of L. rhinoceros cultivar TM02 sclerotia cold water extract (TM-CWE). TM-C WE at 10% (v/v) inhibited melanin production at the intro- and extracellular levels of B16-F1 melanoma cells (50.6-53.3%) and downregulated microplahalinia-associated transcription factor, the key protein for melanogenic enzyme expression, at the intracellular level (44.5 +/- 2.6%). The extract also promoted human procollagen type 1 production in HaCat cells by 51.30 +/- 2.87% at 12.5% (v/v). Irritation tests using three-dimensional eye and skin models further indicated that TM-CWE is a nonirritant with > 75% viability. Therefore, L. rhinoceros TM02 demonstrated the capacity to be a beneficial ingredient for skin care application.
Keywords
Lignosus rhinoceros, Tiger milk mushroom, Pigmentation, Human procollagen type 1, Nonirritant, Melanin, Cosmeceutical compounds, Medicinal mushrooms
Divisions
fac_med
Funders
Fundamental Research Grant FRGS [Grant No: 2019-1 (FRGS/1/2019/SKK08/MAHSA/03/1)],SIRIM Fraunhofer Programme - Enhancement of Competitiveness and Market Access of SMEs (ECMAS) under the SIIMF Fund [Grant No: BIO 238/5/1]
Publication Title
International Journal of Medicinal Mushrooms
Volume
24
Issue
5
Publisher
Begell House Inc
Publisher Location
50 NORTH ST, DANBURY, CT 06810 USA