Improvement of biovanillin production with two-stage pH control strategy from lemongrass leaves hydrolysates using Phanerochaete chrysosporium ATCC 24725 in batch culture
Document Type
Article
Publication Date
7-1-2022
Abstract
The biovanillin production was influenced by varying the culture pH via single control strategy conducted by separate experiments during the fermentation processes. Highest biovanillin production (124 mg/L) with 32% molar yield at culture pH 6.0 from the one-stage control method was observed. Specific growth rates (mu) of thePhanerochaete chrysosporiumand biovanillin production decreased by decreasing the culture pH from 6.0 to 3.5, which indicated that lower culture pH was not adequately apposite for biovanillin production usingPhanerochaete chrysosporiumin a 2-L stirred tank bioreactor. The development of two-stage control strategies had improved the biovanillin production (131 mg/L) and cell concentration (13.0 g/L) by about 6 and 5%, respectively. Therefore, the most influential control strategy for higher biovanillin production was discovered not to control the culture pH of the fermentation during active growth phase of thePhanerochaete chrysosporium, while the production phase should be controlled at pH 6.0.
Keywords
Batch culture, Biovanillin, Lemongrass leaves hydrolysates, Phanerochaete chrysosporiumATCC 24725, pH control strategy, 2-liter stirred tank bioreactor
Divisions
InstituteofBiologicalSciences
Funders
Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Malaysia (Grant No: R.J130000.7945.4s128)
Publication Title
Biomass Conversion and Biorefinery
Volume
12
Issue
7
Publisher
Springer Heidelberg
Publisher Location
TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY