Phenotypic, molecular and serological detection of viral diseases in melon: a comprehensive review on progress and challenges
Document Type
Article
Publication Date
2-1-2025
Abstract
Melons, a highly valued fruit crop, are susceptible to over 40 viruses, mainly belonging to Geminiviradae and Potyviridae. Effective crop protection requires early detection. This review covers recent progress in monitoring and detection methods, encompassing phenotypic, molecular and serological techniques for the detection and identification of viral diseases of melons. PCR-based and ELISA detection methods are widely used for melon. Advanced molecular technology, such as isothermal PCR and CRISPR/Cas systems, has shown great potential to accurately detect viruses even at low concentrations. Additionally, high-throughput sequencing is an innovative technique for identifying unexpected or emerging pathogens, capable of detecting multiple nucleic acids present in infected plants. Recent progress in phenotypical methods using optical remote sensing and machine learning presents significant promise for the non-destructive detection of viruses in melon. Additionally, the advent of new portable devices providing real-time analysis in a short time period is likely to be valuable for in-field detection. The current advancements and application of these methods in plant viral disease monitoring and detection and their potential constraints are discussed.
Keywords
Control strategy, Crop monitoring, Detection, Imaging techniques, Melon, Virus
Divisions
InstituteofBiologicalSciences,cebar
Funders
Project "MRUN: Safety of genetically modified organisms for sustainable agriculture and food" from the Ministry of Education, Malaysia (LR002-2019) ; (UPM/800-4/11/MRUN/2019/5539150)
Publication Title
Journal of Plant Diseases and Protection
Volume
132
Issue
1
Publisher
Springer Heidelberg
Publisher Location
TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY