Detection of Plasmodium knowlesi using recombinase polymerase amplification (RPA) combined with SYBR Green I
Document Type
Article
Publication Date
8-1-2020
Abstract
In this study, recombinase polymerase amplification (RPA) combined with SYBR Green I was developed for the detection of Plasmodium knowlesi. Positive samples were indicated with a green color while negative samples were orange. To increase the efficiency of amplification, an interval mixing step of samples after 3 to 6 min incubation was recommended. Different sets of reaction volumes from 6.25 to 50 mu L were tested and the results indicated no differences in detection. RPA's combination with SYBR Green I is fast and easy to perform, hence this method is suitable for use in resource-limited settings.
Keywords
P. knowlesi, Recombinase polymerase amplification, Isothermal, SYBR Green I, Molecular detection
Divisions
Parasit
Funders
Ministry of Higher Education, Malaysia (Grant No. LR002D-2018)
Publication Title
Acta Tropica
Volume
208
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS