Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria
Document Type
Article
Publication Date
6-1-2020
Abstract
The incidence of zoonotic malaria, Plasmodium knowlesi, infection is increasing and now is the major cause of malaria in Malaysia. Here, we describe a WarmStart colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Plasmodium spp. The detection limit for this assay was 10 copies/mu L for P knowlesi and Plasmodium ovale and 1 copy/mu L for Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae. To test clinical sensitivity and specificity, 100 microscopy-positive and 20 malaria-negative samples were used. The WarmStart colorimetric LAMP was 98% sensitive and 100% specific. Amplification products were visible for direct observation, thereby eliminating the need for post-amplification processing steps. Therefore, WarmStart colorimetric LAMP is suitable for use in resource-limited settings.
Keywords
Borneo, Colorimetry, Humans, Malaria, Nucleic Acid Amplification techniques, Plasmodium knowlesi, Sensitivity and specificity, Species specificity
Divisions
fac_med,Parasit
Publication Title
American Journal of Tropical Medicine and Hygiene
Volume
102
Issue
6
Publisher
American Society of Tropical Medicine and Hygiene
Publisher Location
8000 WESTPARK DR, STE 130, MCLEAN, VA 22101 USA