Nicotiana tabacum as a potential platform for the production of recombinant anti-toxoplasma single-chain variable fragment (scFv) antibody
Document Type
Article
Publication Date
1-1-2020
Abstract
Plant systems have now gained much attention for recombinant therapeutic protein production. In this study, we aimed to evaluate the possibility of producing a single-chain variable fragment antibody (scFv), encoded by the TP60 gene, against Toxoplasmosis disease in Nicotiana tabacum cv. SR1. Toxoplasmosis, caused by infection of a parasitic protozoan known as Toxoplasma gondii, is one of the most prevalent parasitic diseases. Leaf explants of N. tabacum were infected with Agrobacterium tumefaciens strain LBA4404 harbouring binary vector pCAMBIA1304 containing TP60 gene. Bands observed at the predicted size of 914 bp confirmed the presence of TP60 transgene, which was stably integrated into both T0 and T1 tobacco plant genome. FV12-6 transgenic line produced the highest mRNA expression (7-fold) correlated to the highest accumulation of anti- Toxoplasma recombinant scFv antibody (0.52% of the total soluble protein), followed by FV16-10, FV17-7, and FV3-11. Normal growth of the transgenic plants was observed. The segregation analysis of FV3, FV12, and FV16 in T1 generation confirmed the transgene integration within a single locus according to 3:1 Mendelian’s law. These findings indicate the potential of using N. tabacum as a host system to produce TP60 recombinant protein without affecting the normal phenotype of the host plant. © 2020 Malaysian Abstracting and Indexing System. All rights reserved.
Keywords
Plant molecular farming, Plant transformation, Recombinant protein, Single-chain variable fragment antibody (scFv), Tobacco, Toxoplasmosis
Divisions
cebar
Funders
Ministry of Higher Education, High Impact Research Programme (UM.C/625/1/HIR/MOHE/SCI/18),Post-graduate Research Grant of University of Malaya (PG204-2016A),Research University (RU006-2017 and ST003-2018) grants
Publication Title
Malaysian Journal of Science
Volume
39
Issue
2
Publisher
Faculty of Science, University of Malaya