Document Type
Article
Publication Date
1-1-2017
Abstract
This study reports the molecular detection of Theileria spp. from six cattle farms, a sheep farm and a goat farm located at different states in Peninsular Malaysia. Animal b lood samples were screened for the presence of Theileria DNA using a conventional polymerase chain reaction (PCR) assay. A total of 155 (69.2%) of 224 cattle investigated were PCR-positive for Theileria DNA. The occurrences of Theileria spp. ranged from 17.5% to 100.0% across six cattle farms. Theileria DNA was detected from 90.0% of 40 sheep but none of 40 goats examined in this study. Sequence analyses of amplified 18S rRNA partial fragments (335-338 bp) confirmed the identification of Theileria buffeli, Theileria sergenti, and Theileria sinensis in representative samples of cattle and ticks. T. luwenshuni was identified in the infected sheep. The high occurrences of Theileria spp. in our farm animals highlight the needs for appropriate control and preventive measures for theileriosis.
Keywords
Theileria spp., Cattle, Sheep, Peninsular Malaysia
Divisions
fac_med
Funders
University of Malaya: High Impact Research Chancellery Grant (UM.C/625/1/HIR/CHAN/11), and UMRG Grant (RP013-2012A),Ministry of Science, Technology and Innovation E-Science Fund (SF014-2015)
Publication Title
Veterinary Parasitology
Volume
10
Publisher
Elsevier
Event Title
26th International Conference of The World Association for the Advancement of Veterinary Parasitology
Event Location
KLCC, Kuala Lumpur, Malaysia
Event Dates
04-08 September 2017
Event Type
conference