Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
Document Type
Article
Publication Date
1-1-2017
Abstract
Objective To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates. Methods Peripheral blood, bone marrow and spleen aspirate samples were collected from clinically suspected VL patients (n = 26). A new PCR primer pair (MK1F/R) was designed targeting kinetoplast mini circle DNA sequences of Leishmania donovani, and Leishmania infantum, and was used to diagnose VL along with some other established primers for VL in polymerase chain reactions. Test was validated by comparing with several other diagnostic methods. Results The designed primer set showed 100% specificity and 98% sensitivity in detecting VL using blood samples, when compared with more invasive samples: bone marrow or spleen aspirates. Conclusions The newly designed primer MK1F/R could be a better alternative for PCR based diagnosis of VL using less invasive sample, peripheral blood instead of bone marrow or spleen aspirates.
Keywords
Visceral leishmaniasis, Kala-azar, Leishmania species, PCR detection, New primer
Divisions
fac_med
Funders
Bangladesh Medical Research Council (BMRC), (Reference number BMRC/HPNSDP/Research Fund/2012–2013/3449344(34), Dated, 20 March 2013)
Publication Title
Asian Pacific Journal of Tropical Medicine
Volume
10
Issue
8
Publisher
Elsevier