Document Type
Article
Publication Date
2-1-2015
Abstract
High Pressure Processing of Royal Gala apple, Taylor's Gold pear and Camarosa strawberry purees was carried out to inactivate polyphenoloxidase (PPO). First, 600 MPa enzyme inactivation at room temperature was investigated. After 60 min, strawberry (8 RA, residual activity) and apple (89 RA) PPOs were partially inactivated and pear PPO was not inactivated, demonstrating the high variability in the resistance of different fruits' PPOs. Then, the fruit purees were submitted to 600 MPa combined with mild heat, and the PPO inactivation kinetics was modeled. The pear PPO was found to be resistant even after 60 min 600 MPa-71 degrees C process. Regarding apple and strawberry PPOs, 600 MPa-thermal inactivation of PPO followed a biphasic first order kinetics exhibiting stable and labile fractions. The k(s) values (rate constant for stable fraction) at 57 degrees C and 71 degrees C were 0.0121 min(-1) and 0.0184 min(-1) for apple PPO, and 0.0182 min(-1) and 0.0805 min(-1) for strawberry PPO. (C) 2014 Elsevier Ltd. All rights reserved.
Keywords
Browning, polyphenoloxidase, enzyme, high pressure processing, high hydrostatic pressure, kinetics, high hydrostatic-pressure, ultra-high pressure, pyrus-communis l, vegetable products, victoria grape, oxidase, quality, enzymes, purification, temperature
Divisions
fac_eng
Funders
Bright Sparks Program, University of Malaya, Kuala Lumpur, Malaysia ,Faculty Research Development Fund, Faculty of Engineering, University of Auckland, New Zealand
Publication Title
Journal of Food Engineering
Volume
147
Additional Information
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