Effects of even and odd number fatty acids cofeeding on PHA production and composition inPseudomonas putidaBet001 isolated from palm oil mill effluent
Document Type
Article
Publication Date
1-1-2015
Abstract
The biosynthesis of medium-chain-length poly-3-hydroxyalkanoates by Pseudomonas putida Bet001 cultivated on mixed carbon sources was investigated. The mixed carbon sources consisted of heptanoic acid (HA) and oleic acid (OA). A relatively low PHA content at 1.2% (w/w) and 11.4% (w/w) was obtained when HA or OA was used as the sole carbon source. When these fatty acids were supplied as a mixture, PHA content increased threefold. Interestingly, the mixture-derived PHA composed of both odd and even monomer units, namely. 3-hydroxyheptanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydroxydodecanoate and no unsaturated monomer was detected. It is hypothesized that the even-numbered monomers were derived primarily from OA, whereas the odd-numbered monomer was derived from HA. This also points out to an efficient and yet distinct fatty acids metabolism that fed the PHA biosynthesis machinery of this particular microorganism. PHA obtained was elastomeric because melting temperature (Tm) and crystallinity were absent. It showed good thermal stability with degradation temperature (Td) ranging from 275.96 to 283.05 °C.
Keywords
Heptanoic acid, Odd and even monomers, Oleic acid poly-3-hydroxyalkanoates, Pseudomonas putida
Divisions
InstituteofBiologicalSciences
Publication Title
Biotechnology and Applied Biochemistry
Volume
63
Issue
1
Publisher
Wiley