A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity
Document Type
Article
Publication Date
1-1-2015
Abstract
Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39 μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97 × 104 M-1. Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway.
Keywords
Thiosemicarbazone, Fluorescent, Chemosensor, DNA binding, Chemical nucleases
Divisions
CHEMISTRY,PHYSICS
Funders
University Malaya: University of Malaya Research Grant (RP025A-14AFR), High Impact Research Grant (J-21002-73851) and Postgraduate Research Grant (PG105-2012B),University Malaya: Bright Sparks fellowship (BSP/APP/0944/2013)
Publication Title
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Volume
150
Publisher
Elsevier