A cell-based screening system for anti-influenza A virus agents
Document Type
Article
Publication Date
1-1-2015
Abstract
Emerging of drug resistant influenza A virus (IAV) has been a big challenge for anti-IAV therapy. In this study, we describe a relatively easy and safe cell-based screening system for anti-IAV replication inhibitors using a non-replicative strain of IAV. A nickel (II) complex of polyhydroxybenzaldehyde N4-thiosemicarbazone (NiPT5) was recently found to exhibit anti-inflammatory activity in vivo and in vitro. NiPT5 impedes the signaling cascades that lead to the activation of NF-κB in response to different stimuli, such as LPS and TNFα. Using our cell-based screening system, we report that pretreating cells with NiPT5 protects cells from influenza A virus (IAV) and vesicular stomatitis virus (VSV) infection. Furthermore, NiPT5 inhibits replication of IAV by inhibiting transcription and translation of vRNAs of IAV. Additionally, NiPT5 reduces IAV-induced type I interferon response and cytokines production. Moreover, NiPT5 prevents activation of NF-κB, and IRF3 in response to IAV infection. These results demonstrate that NiPT5 is a potent antiviral agent that inhibits the early phase of IAV replication.
Keywords
Antiviral Agents, Cell Line, Tumor, Cytokines, Drug Evaluation, Preclinical, Humans, Influenza A virus, Interferon Regulatory Factor-3, Interferons, NF-kappa B, Virus Replication
Divisions
fac_med,CHEMISTRY,InstituteofBiologicalSciences
Publication Title
Scientific Reports
Volume
5
Issue
1
Publisher
Nature Publishing Group