Document Type
Article (Restricted)
Publication Date
1-1-2012
Abstract
In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (�200 bp) from 21 of 310 (6.8) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the 'zoonotic' assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia.
Keywords
Giardia duodenalis Small subunit of the nuclear ribosomal RNA (SSU) gene Goats Malaysia
Divisions
Parasit
Publication Title
Molecular and Cellular Probes
Publisher
Elsevier
Additional Information
Department of Parasitology, Faculty of Medicine University of Malaya, 50603 Kuala Lumpur, MALAYSIA