Spermiogenesis and spermatozoon of the tapeworm Parabothriocephalus gracilis (Bothriocephalidea): Ultrastructural and cytochemical studies

Document Type

Article

Publication Date

1-1-2010

Abstract

Spermiogenesis and spermatozoon ultrastructure of the tapeworm Parabothriocephalus gracilis were described using transmission electron microscopy (TEM). Spermiogenesis is characterized by the formation of a zone of differentiation with two centrioles associated with striated rootlets, and an intercentriolar body between them. The two flagella undergo a rotation of 90 until they become parallel to the median cytoplasmic extension with which they fuse. Electron-dense material is present in the apical region of the zone of differentiation in the early stages of spermiogenesis. This electron-dense material is characteristic for the orders Bothriocephalidea and Diphyllobothriidea. The mature spermatozoon contains two axonemes of the 9 + `1' trepaxonematan pattern, nucleus, parallel cortical microtubules and electron-dense granules of glycogen. The anterior extremity of the spermatozoon exhibits a single helical electron-dense crested body 130 nm thick. One of the most interesting features is the presence of a ring of cortical microtubules surrounding the axoneme. This character has been reported only for species of the order Bothriocephalidea and may be unique in this cestode group.

Keywords

Eucestoda, Bothriocephalidea, Parabothriocephalus gracilis, spermiogenesis, spermatozoon, ultrastructure

Publication Title

Acta Parasitologica

Volume

55

Issue

1

Publisher

Versita

Publisher Location

9 DRUGA POPRZECNA ST, 04-604 WARSAW 41, POLAND

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