Document Type
Article
Publication Date
1-1-2008
Abstract
This paper presents the first reported use of 18S rRNA gene sequence to determine the phylogeny of Brugia pahangi. The 18S rRNA nucleotide sequence of a Malaysian B. pahangi isolate was obtained by PCR cloning and sequencing. The sequence was compared with 18S rRNA sequences of other nematodes, including those of some filarial nematodes. Multiple alignment and homology analysis suggest that B. pahangi is closely related to B. malayi and Wuchereria bancrofti. Phylogenetic trees constructed using Neighbour Joining, Minimum Evolution and Maximum Parsimony methods correctly grouped B. pahangi with other filarial nematodes, with closest relationship with B. malayi and W. bancrofti. The phylogeny of B. pahangi obtained in this study is in concordance with those previously reported, in which the 5S rRNA gene spacer region and cytochrome oxidase subunit I (COI) sequences were used.
Keywords
Parasitology
Publication Title
Tropical Biomedicine
ISSN
0127-5720
Recommended Citation
Fong, M.Y.; Thanabalan, A.; Muslim, A.; Lau, Y.L.; Sivanandam, S.; and Mahmud, R., "Inferring the phylogenetic position of Brugia pahangi using 18S ribosomal RNA (18S rRNA) gene sequence" (2008). Research Publications (2006 to 2010). 1492.
https://knova.um.edu.my/research_publications_2006_2010/1492
Divisions
fac_med
Volume
25
Issue
1
Additional Information
Parasitology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia E-mail: fongmy@um.edu.my