Document Type
Article (Restricted)
Publication Date
1-1-2008
Abstract
The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80 (IgG negative, IgM positive) to 100 (IgG positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii.
Keywords
Pichia Pastoris, Recombinant SAG2 Antigen, Toxoplasma Gondi
Divisions
fac_med
Publication Title
Biotechnology Letters
Volume
30
Issue
4
Publisher
Kluwer (now part of Springer)
Additional Information
Fong, Mun Yik Lau, Yee Ling Zulqarnain, Mohamed