Document Type
Article (Restricted)
Publication Date
1-1-2004
Abstract
Binding of dengue virus 2 (DENV-2) to C6/36 mosquito cells protein was investigated. A 48 kDa DENV-2-binding C6/36 cells protein (D2BP) was detected in a virus overlay protein-binding assay. The binding occurred only to the C6/36 cells cytosolic protein fraction and it was inhibited by free D2BP. D2BP was shown to bind to DENV-2 E in the far-Western-binding studies and using mass spectrometry (MS) and MS/MS, peptide masses of the D2BP that matched to beta-tubulin and alpha-tubulin chains were identified. These findings suggest that DENV-2 through DENV-2 E binds directly to a 48 kDa tubulin or tubulin-like protein of C6/36 mosquito cells. (C) 2004 Elsevier Inc. All rights reserved.
Keywords
Amino Acid Sequence Animals Binding Sites Cell Line Culicidae/*metabolism/*virology Dengue Virus/*metabolism Insect Proteins/*chemistry/metabolism Molecular Sequence Data Molecular Weight Protein Binding Protein Interaction Mapping/*methods Sequence Alignment Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods Tubulin/*chemistry/metabolism Viral Envelope Proteins/*chemistry/metabolism
Divisions
fac_med
Publication Title
Biochemical and Biophysical Research Communications
Volume
320
Issue
1
Additional Information
Chee, HY AbuBakar, S