Document Type
Article
Publication Date
1-1-2001
Abstract
Pulsed field conditions specific for Pasteurella multocida B:2 have been optimised to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200 V, ramped pulsed time of 1-25 s and a run time of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.
Keywords
Pasteurella multocida, PFGE, restriction enzymes
Divisions
InstituteofBiologicalSciences
Publication Title
Asia Pacific Journal of Molecular Biology and Biotechnology
Volume
9
Issue
2
Publisher
Malaysian Society for Molecular Biology and Biotechnology
COinS