Document Type
Article
Publication Date
1-1-2001
Abstract
Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V, ramped pulse time of 1-25 s, and a runtime of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.
Keywords
Pasteurella multocida, PFGE, restriction enzymes
Publication Title
Asia Pacific Journal of Molecular Biology and Biotechnology
ISSN
0128-7451
Recommended Citation
Hasima, N.N.; Saidi, J.; Thong, Kwai Lin; and Ansary, A., "Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA" (2001). Research Publications (2000 to 2005). 316.
https://knova.um.edu.my/research_publications_2000_2005/316
Divisions
InstituteofBiologicalSciences
Volume
9
Issue
2
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Additional Information
Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA