Document Type

Article (Restricted)

Publication Date

9-12-2005

Abstract

Muscarinic receptor binding activity was tested on 224 plant extracts obtained from more than 50 plant families found in Malaysia. The plant extracts were evaluated by a 96-well microplate filtration–based radioligand competitive assay, centered on the ability of the plant extracts to competitively displace the radioligand, [3 H]N-methylscopolamine, from binding to the muscarinic membrane receptors. The screening assay was initially carried out at 50 mg=assay point, and those showing inhibition at and above 61% were retested at 10 mg=assay point. The extracts of Ficus septica Burm. f. (Moraceae) [65.85 � 3.75% inhibition; mean (n ¼ 3) � SD], Polyalthia microtus Miq. (Annonaceae) (32.63 � 1.38% inhibition), and Popowia odoardoi Diels (Annonaceae) (35.79 � 7.11% inhibition) at 10 mg=assay point exhibited muscarinic properties, which are worthy of further investigation.

Keywords

Ficus Septica, Malaysian Plants, Muscarinic Receptor, Polyalthia Microtus, Popowia Odoardoi, Radioligand Binding Assay

Publication Title

Pharmaceutical Biology

ISSN

1388-0209

Divisions

fac_med

Volume

43

Issue

8

Publisher

Taylor & Francis

Additional Information

Address correspondence to: L.Y. Chung, Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. E-mail: chungly@hotmail.com

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