Document Type
Article (Restricted)
Publication Date
9-12-2005
Abstract
Muscarinic receptor binding activity was tested on 224 plant extracts obtained from more than 50 plant families found in Malaysia. The plant extracts were evaluated by a 96-well microplate filtration–based radioligand competitive assay, centered on the ability of the plant extracts to competitively displace the radioligand, [3 H]N-methylscopolamine, from binding to the muscarinic membrane receptors. The screening assay was initially carried out at 50 mg=assay point, and those showing inhibition at and above 61% were retested at 10 mg=assay point. The extracts of Ficus septica Burm. f. (Moraceae) [65.85 � 3.75% inhibition; mean (n ¼ 3) � SD], Polyalthia microtus Miq. (Annonaceae) (32.63 � 1.38% inhibition), and Popowia odoardoi Diels (Annonaceae) (35.79 � 7.11% inhibition) at 10 mg=assay point exhibited muscarinic properties, which are worthy of further investigation.
Keywords
Ficus Septica, Malaysian Plants, Muscarinic Receptor, Polyalthia Microtus, Popowia Odoardoi, Radioligand Binding Assay
Divisions
fac_med
Publication Title
Pharmaceutical Biology
Volume
43
Issue
8
Publisher
Taylor & Francis
Additional Information
Address correspondence to: L.Y. Chung, Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. E-mail: chungly@hotmail.com