Date of Award
1-1-2011
Thesis Type
masters
Document Type
Thesis
Divisions
science
Department
Institute of Biological Sciences
Institution
University of Malaya
Abstract
The primary role of tumor necrosis factor alpha (TNF-α) gene is to regulate immune cells. Dysregulation and, in particular, overproduction of this gene has been found to increase susceptibility to a variety of human diseases such as cancer. The aim of this study is to investigate the association of single nucleotide polymorphism (SNP) in TNF-α −308 promoter and the risk of oral cancer among the Malaysian Indian and Indigenous population.The study included 143 confirmed oral squamous cell carcinoma (OSCC) (mean age = 63.69 ± 12.84) and 79 healthy controls (mean age = 50.43 ± 16.35). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to analyze TNF-α −308 promoter polymorphism, which were confirmed by direct sequencing. Chi-square, simple logistic regression and stratified analysis were performed using the SPSS (ver 15.0) to study the role of TNF-α polymorphism in modulating the risk of oral cancer. The wild-type genotype (GG) was seen in 88.8% (127) of OSCC patients in comparison to 87.3% (69) controls; while variant genotypes (GA & AA) were seen in 9.8% (14) and 1.4% (2) of cases and 11.4% (9) and 1.3% (1) of controls respectively. Also no significant association was observed between variant genotypes (GA & AA) and oral cancer risk. Polymorphism of TNF-α at position −308 G/A may not be a risk factor for oral cancer because we did not find a statistically significant association between the TNF-α −308 G/A polymorphism and oral cancer risk (p = .710 and p = .946 for GA and AA respectively). In conclusion, no association was seen between TNF-α −308 G/A polymorphism and oral cancer risk among the Malaysian Indian and Indigenous population.
Note
Dissertation submitted in partial fulfillment of the requirement for the degree of Master of Biotechnology
Recommended Citation
Golpich, Mojtaba, "Association between TNF-a-308 G/A polymorphism and oral cancer risk among Malaysian Indian and indigenous / Mojtaba Golpich" (2011). Student Works (2010-2019). 640.
https://knova.um.edu.my/student_works_2010s/640