Date of Award

8-1-2017

Thesis Type

masters

Document Type

Thesis (Restricted Access)

Divisions

science

Department

Faculty of Science

Institution

University of Malaya

Abstract

Ten compounds were successfully isolated from the dichloromethane extract of bark of Walsura pinnata Hassk which was collected at 243 km, from Gua Musang, Kelantan to Kuala Lipis, Pahang. A new oleanane triterpenoids; 3-oxo-olean-9(11),12-dien-28-oic acid (100) was established along with nine known compounds, which are five terpenoids, 3-oxo-lup-20(29)-en-28-oic acid (97), 3β-hydroxy-5-glutinen-28-oic acid (98), 3-oxo-olean-11-en-28,13β-olide (99), 3-oxo-20(24)-epoxy-12β,25-dihydroxydammarane (101), 2(3),6(7)-diepoxy-9-humulene (102), two sterols compounds, stigmasterol (103), β-sitosterol glycoside (104) with two aromatic derivatives, which are 4-hydroxy-3-methoxybenzoic acid (105) and 4-hydroxy-4,8-dimethyl-1-tetralone (106) were analyzed and confirmed using various spectroscopic techniques. Compound 97 and 98 were tested in vitro for their cytotoxic activities against five type of cancer cell lines; human hepatocellular carcinoma (Hep3b and HepG2), human ovarian adenocarcinoma (SK-OV-3), human breast adenocarcinoma (MCF-7), and human acute myeloblastic leukemia (Kasumi-1). Compound 97 showed good cytotoxicity against leukemia, Kasumi-1 cell line with IC50 value of 2.11 ± 0.48 μg/mL while compound 98 exhibit good cytotoxicity activities against both liver cancer cell lines, Hep3b (IC50: 2.28 ± 0.00 μg/mL , SI = 8.81) and HepG2 (IC50: 4.00 ± 0.63 μg/mL, SI = 5.02).

Note

Dissertation (M.A.) – Faculty of Science, University of Malaya, 2017.

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