Date of Award

4-1-2017

Thesis Type

masters

Document Type

Thesis

Divisions

medic

Department

Faculty of Medicine

Institution

University of Malaya

Abstract

Human papillomavirus (IIPV) infection and its associated cancers disproportionately affect those infected with human immunodeficiency virus (HIV) despite effective combination antiretroviral therapy (cART). Cell-mediated immune (CMI) responses against HPV E6 antigen have been associated with clearance of HPV infection or its associated diseases. This study aims to investigate the presence of IIPV 16 and 52 E6- specific T cell responses in HIV+ patients on cART with suboptimal (siR) and optimal (olR) immune reconstitution. A cross-sectional cohort (n= 67) consisting of patients with siR (CD4+ T cell <350cells/mm3) and oiR (CD4+ T cell >500cells/mm3) after a minimum of2-years on cART with sustained suppressive viral load RNA <50copies/ml over a year following cART were recruited from Infectious Diseases clinic in University of Malaya Medical Centre (UMMC). A detailed questionnaire on socio-demographic information was administered. Specimens obtained from each participant included whole blood, an anal swab and oral rinse. HPV DNA genotyping test was performed on anal and oral specimens. Anal cytology was also undertaken. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood for interferon (IFN)-y ELISPOT and flow cytornetry. IFNy ELISPOT was used to detected IIPV16 and IIPV52 E6-specific T cell immune responses whereas flow cytometry was to identify the immunophenotypes of CD4+ and CD8+T cells at systemic level. All IllY+ individuals received non-nucleoside reverse transcriptase inhibitors (NNRTI)-based cART. As compared to olR group, siR group exhibited lower baseline of CD4+ T cell count (28 vs 190cells/mm3, p

Note

Dissertation (M.A.) – Faculty of Medicine, University of Malaya, 2017.

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