Date of Award

1-1-2014

Thesis Type

masters

Document Type

Thesis

Divisions

science

Department

Department of Biological Science Faculty Of Science

Institution

University of Malaya

Abstract

CG16936, which was classified as DmGSTE12 was studied in this project. This study cloned and expressed CG16936 and the resulting recombinant CG16936 has been reactive towards 1,2-dichloro-4-nitrobenze (DCNB) (0.4324 μmol/min/mg) but of no activities towards, 1-chloro-2,4-nitrobenzene, trans-2-hexenal, p-nitrobenzyl chloride (NBC), ethacryanic acid (EA), trans-4-phenyl-butene-2-one (PBO), hexa-2,4-dienal, trans,trans-hepta-2,4-dienal, 1,2-epoxy-3-p-nitrophenoxy propane (EPNP), bromosulfophthalein (BSP) and trans-oct-2-enal. In previous work, it was shown that CG16936 was expressed in response to odorant treatment to fruit flies. Our work investigated the direct contribution of the recombinant CG16936 towards conjugation of odorants Glutathione (GSH). No evidence of conjugated product yielded when the recombinant protein acted upon GSH and trans-2-hexenal on thin layer chromatography. The absence of enzymatic activity hence suggested the inability of CG16936 to conjugate t-2-hexenal to GSH. Upon exposure to benzaldehyde, expression of CG16936 was demonstrated unchanged. The evidences suggested that CG16936 could have assumed other physiological function other than detoxification of odorant and its direct involvement in oxidative stress.

Note

Dissertation (M.A) - Department of Biological Science Faculty of Science, University of Malaya, 2013.

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