Date of Award

1-1-2010

Thesis Type

masters

Document Type

Thesis

Divisions

science

Department

Institute of Biological Sciences

Institution

University of Malaya

Abstract

Mitochondrial DNA (mtDNA) markers has been widely used to better resolve the taxonomic identification as well as display the genetic relatedness of different species and closely related fish. The highly cost ornamental fish, Asian arowana (Scleropages formosus) of family Osteoglossidae has different colored strains that are geographically distributed in different regions around Southeast Asia. The taxonomic classification of these different strains still remains unclear and disputable. The partial sequences of the cytochrome b and the DNA barcoding gene, cytochrome C oxidase were analyzed and assessed for molecular identification; to differentiate the colored varieties of this species. The results of this study indicated that there was lack of both COI and cytochrome mtDNA differentiation among the red and golden main varieties that it did not indicate emergence of new species. However, the partial sequences of the genes are sufficient to identify the species when compared to its closely related species as well as the green strains compared to other strains of the Asian arowana. In the future, more markers will be used to obtain a more concrete evidence to support the existence of a relationship among Arowana starians.

Note

Dissertation submitted in fulfillment of the requirement for the degree of Master of Biotechnology

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