Date of Award

2010

Thesis Type

Masters

Document Type

Dissertation

Divisions

Faculty of Science

Department

Institute of Biological Sciences

Institution

Universiti Malaya

Abstract

The rhizomes of the Zingiberaceae family are vegetables widely used in many Asian countries and their medicinal functions have been broadly discussed and accepted in many traditional recipes. In this study, the antimicrobial activity of extracts of Curcuma zedoaria from Malaysia was compared between two systems as in vitro and in vivo. The comparison was performed against four bacterial strains including two gram negative strains (E.coli and Pseudomonas aeruginosa) and two gram positive strains (Bacillus cereus and Staphylococcus aureus) using the agar well diffusion method. Extracts were made in three different solvents; petroleum ether, chloroform and methanol extracts exhibited antibacterial activity. Growth hormones, Indole-3-butyric acid (IBA) and 6- Benzylaminopurine (BAP) were used in the in vitro system separately between the range of 0.25-3.5 mg/ l for IBA and 0.5-4 mg/l for BAP and a mix formula containing both hormones using the best ranges after rapid tests. Petroleum ether extracts showed great comparable antimicrobial results with the in vitro system for Bacillus cereus and Staphylococcus aureus as well as methanolic extracts did. Chloroform extracts showed comparable antimicrobial activity for both Bacillus cereus and Staphylococcus aureus. This study is the first report of the antimicrobial comparison between in vitro and in vivo systems of Curcuma zedoaria in Malaysia. Curcuma zedoaria has shown antimicrobial activity both in vitro and in vivo systems based on the results presented in this study.

Initial

khm

Additional Information

Dissertation (M.A.) – Faculty of Science, Universiti Malaya, 2010.

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