Date of Award

2010

Thesis Type

Masters

Document Type

Dissertation

Divisions

Faculty of Science

Department

Institute of Biological Sciences

Institution

Universiti Malaya

Abstract

Studies were conducted to develop the tissue culture protocol and mutagenesis through Gamma ray irradiation for two variegated Sansevieria species known as Sansevieria trifasciata var. Laurentii (goldband Sansevieria) and Sansevieria trifasciata var. Hahnii (birdnest Sansevieria). Seven combinations of BAP and NAA were chosen which are represented by letter A= 1.00 mg/I BAP, B = 1.00 mg/I BAP + 0.25 mg/I NAA, C = 2.00 mg/I BAP + 0.50 mg/I NAA, D = 2.00 mg/I BAP + 1.00 mg/I NAA, E = 3.00 mg/I BAP + 1.00 mg/I NAA, F = 4.00 mg/I BAP + 1.00 mg/I NAA and G = 5.00 mg/I BAP + 2.00 mg/I NAA. The best result for shoot numbers in variety Hahnii was obtained by using explants incubated on Murashige and Skoog ( 1962) induction media with higher BAP compared to NAA but this was not consistent for variety Laurentii. Maximum shoot elongation in both varieties were achieved when MS media containing 5.00 mg/I BAP with 2.00 mg/I NAA and 4.00 mg/1 BAP with 1.00 mg/1 NAA were used. For the next stage, elongated shoots were excised and rooted best on MS modified medium containing l.0Omg/1 IAA growth regulator in light condition (3.00 for Hahnii and 2.83 for Laurentii). However, as for root length, it showed the best result on media containing 2.00 mg/I IAA in light conditions for both Sanseveria. The two varieties of Sansevieria showed that there is significant difference between the longest roots produced by variety Hahnii (3.73±0.31) and variety Laurentii (7 .08±0.55). Rooted plantlets were acclimatiz.ed under greenhouse conditions. The explants which were irradiated with low Gamma irradiation (0 to 25 Gy for variety Hahnii and 0 to 30 Gy for variety Laurentii) produced longer shoots than those irradiated with a high dose (more than 40 Gy). A significant reduction in the survival (12.5%) of the Sansevieria explants was observed following treatment with 25 Gy or higher ii doses of Gamma ray. Variety Laurentii (LD50 = 44 Gy) was found to be less sensitive to Gamma irradiation as compared to variety Hahnii (LD50 = 38 Gy). All explants exposed to Gamma rays above 100 Gy dose did not survive. Variety Hahnii exhibited better growth and rigid leaf structure than variety Laurentii during acclimatization.

Initial

khm

Additional Information

Dissertation (M.A.) – Faculty of Science, Universiti Malaya, 2010.

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