Kim Kee Tam

Date of Award

1-1-2006

Thesis Type

masters

Document Type

Thesis

Department

Faculty of Medicine

Institution

University of Malaya

Abstract

The present study aims to study the pathogenesis of mice-adapted dengue type 2 virus (DENV-2MA) in an adult mice model towards the development of a potential animal model for dengue. This was performed by first adapting DENY- 2 in suckling mice brain, determining the virus viraemia phase and virus tropism in the infected adult mice, and evaluating specific immune response of the infected mice against the DENY-2MA and un"d" apted dengue virus type 2 (DENY- \ 2uA)· For the adaptation of DENV-2 in suckling mice brain, DENY-2 was inoculated into 1 day old suckling mice brain and harvested at day 7 postinfection (p.i). This was repeated three times prior to inoculation of the DENV-2MA into adult mice. Adult BALB/c mice (4 to 5 weeks) were infected intraperitoneally with 1.0 x 103 foci forming unit (Ifu) D ... NV-2MA or D NV-2u to study the virus replication in adult mice. Our finding showed that no iraemia phase was detected in these BALB/c mice. DENV-2MA, however, was r co ered from the infected mice brain and spleen. Study on the DENV-2MA and D NV- 2uA infected mice immune response showed that both DENY-2MA and D NV-2u were able to elicit primary immune response and anarnne tic re pon e in mice. The mice group that were inoculated with DENY-2MA and challenged with DENV-2uA gave the highest IgG level in comparison to other mice group . Our results revealed that DENV-2MA replicates better and elicits better immun response in adult BALB/c mice when compared with D NV-2u . The D NV- 2MA, hence, makes a more uitable candidate for the tud of D NV infe tion in II adult mice and ought to be used for future development of an animal model for DENY infection.

Note

Dissertation (M.A.) Faculty of Medicine, University of Malaya, 2006.

Download

Share

COinS