Date of Award

1-1-2000

Thesis Type

Masters

Document Type

Thesis

Divisions

Institute for Advanced Studies

Department

-

Institution

Universiti Malaya

Abstract

An inexpensive carrier matrix. vermiculite was used for immobilizing urease. A cost effective and simple method of coupling was developed using vermiculite. Immobilization of urease on Amberlite MB-land Nylon-6 tube were also studied. Activities of free and immobilized urease were studied and compared: Among all methods used for immobilization of urease. the method of coupling the enzyme to vermiculite gave the highest retained activity of 82% (Method I) and 89% (Method 2) at pH 6.0 and 5.5 respectively. Urease immobilized by Method I retained 69% and 30% of its original activity and Method 2 (using glutaraldehyde as coupling agent) retained 81% and 45% of its original activity after 60 days when stored in phosphate buffer at 4°C and 25°C respectively. Urease immobilized by these methods retained 68% and 57% of its original activity respectively after 5 repeated use. Urease was also immobilized on nylon-6 using glutaraldehyde as coupling agent. Urease immobilized by this method retained 76% and 51. 7% after 60 days when stored at 4°C and 25°C respectively. It retained 78% of its original activity after 5 repeated use. The immobilized urease obtained by coupling urease to Amberlite MB-I using glutaraldehyde had a retained activity of 59% at pH 6.0. Urease immobilized by this method retained 62% and 25.3% of its original activity after 60 days when stored at 4°C and 2s0c respectively. It retained 68% of its original activity after 5 repeated operations.

Initial

snms

Additional Information

Dissertation (M.A) -- Institute for Advanced Studies, Universiti Malaya, 2000

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