Method development and validation for drug analysis by HPLC GC-MS

Author

• Yann jen Goh, Universiti Malaya

Date of Award

1-1-2000

Thesis Type

Masters

Document Type

Thesis

Divisions

Faculty of Science

Department

-

Institution

Universiti Malaya

Abstract

Enalapril and its metabolite Enalaprilat were determined by gas chromatography - mass spectrometry (GC - MS) and high - performance liquid chromatography (HPLC). For HPLC, the detection limit of enalapril and enalaprilat was 10n g/ml in methanol, whereas for the GC - MS, the detection limit was 6.25ng/ml in plasma. These methods were compared and GC - MS was selected for routine analysis. Enalapril and Enalaprilat in plasma were extracted and cleaned up by using Sep - Pak C 18. Enalapril and Enalaprilat were detected after reaction with diazomethane and were identified by gas chromatography - mass spectrometry as methyl ester. Detection by selected ion monitoring (SIM) was selected to m/z 220 (enalaprilat) and m/z 234 (enalapril). Diazepam was used as an internal standard. This method is applied to the pharmacokinetic analysis of enalapril and enalaprilat in body fluids.

Initial

snms

Additional Information

Dissertation (M.A) -- Faculty of Science, Universiti Malaya, 2000.

Recommended Citation

Goh, Yann jen, "Method development and validation for drug analysis by HPLC GC-MS" (2000). Student Works (2000-2009). 166.
https://knova.um.edu.my/student_works_2000s/166

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.