Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen

Document Type

Article

Publication Date

1-1-1995

Abstract

Cattle major histocompatibility complex (MHC) (BoLA) class I molecules are heterodimeric glycoproteins which present endogenous antigenic peptides to CD8{sup +} T lymphocytes, initiating a cellular immune response. The MHC-encoded heavy chains are highly polymorphic and, in cattle, have been characterized mainly by using allo-antisera raised by reciprocal calf/dam immunizations. This has enabled the identification of about 50 serlogical specificities, most of which behave as alleles of a single highly polymorphic class I locus. However, evidence from biochemical and molecular biological studies suggest that more than one BoLA class I locus is expressed. These loci are apparently in linkage disequilibrium, making them difficult to distinguish by conventional methods. In order to investigate the expression and function of individual class I locus products, we are correlating BoLA class I gene sequences with the expressed products by the transfection and characterization of genomic class I clones. Shotgun transfection and expression of BoLA class I molecules has been described previously, but the genes involved were not isolated. In this paper we report the isolation, DNA sequencing, transfection, and expression of a genomic clone encoding a BoLA-A11 determinant from an animal expressing A10 and A11 serological specificities. 23 refs., 3 figs.

Keywords

Amino acid sequence, Animals, Base sequence, Cattle, Gene expression, Genetics, Genes, Molecular sequence data, Transfection

Publication Title

Immunogenetics

ISSN

0093-7711

Divisions

InstituteofBiologicalSciences

Volume

41

Issue

4

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