Antioxidant and cytotoxicity activities of butylated hydroxytoluene ligands capped gold nanoparticles
Document Type
Article
Publication Date
1-1-2021
Abstract
The new potent nano-antioxidant of sulfur-containing butylated hydroxytoluene ligands (S-BHTLs)-conjugated with gold nanoparticles, Au-S-BHTLs, was synthesized by a conjugation of sulfur-containing ligands derived from BHT on the surface of gold nanoparticles (AuNPs). The in-house developing eight sulfur-containing BHT-ligands (S-BHTLs) were used for further study on functionalization with AuNPs and their biological activities. The antioxidant and cytotoxic abilities of eight types of Au-S-BHTLs such as compound Au-2a, Au-2b, Au-3a, Au-3b, Au-4a, Au-4b, Au-5a and Au-5b were tested by DPPH center dot(2,2-diphenyl-1-picrylhydrazyl) radical scavenging and MTT assays against HT29 (human colorectal adenocarcinoma) and MCF7 (human breast adenocarcinoma). Based on the results, it was revealed that nanocomposites Au-5a and Au-3a attained over 50% lower IC50 values against free radical than the un-functionalized AuNPs. Meanwhile, nanocomposite Au-2b showed the highest cytotoxicity effect against both cancer cell lines. Selectivity and safety towards non-tumorgenic cell lines were also evaluated and proved superior selectivity index (SI) of all nanocomposites (SI > 2.00) against both cancer cells except Au-5b (SI = 1.87) against MCF7. Hence, the functionalization of S-BHTLs with gold nanoparticles has increased the selectivity of nanocomposites and enhanced the antioxidant potentials to become useful and promising anticancer agents.
Keywords
Gold nanoparticles, Au-S-BHTLs, DPPH, HT29, MCF7
Funders
Ministry of Education, Malaysia (FP050-2014B) (FP127-2019A),Universiti Malaya (PPP-2015B)
Publication Title
Chiang Mai Journal of Science
Volume
48
Issue
2
Publisher
Faculty of Science, Chiang Mai University
Publisher Location
239 HUAY KAEW RD, T SUTHEP, CHIANG MAI, 50200, THAILAND