Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
Document Type
Article
Publication Date
8-18-2022
Abstract
Background High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. Methods A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment. Results Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3-96.3%) and 93.6% specificity (95% CI 87.3-97.4%) of RT-LAMP. Conclusions The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool.
Keywords
Diagnosis, RT-LAMP, SARS-CoV-2, Phenol red, pH sensitive indicator
Divisions
Parasit
Funders
Prototype Research Grant Scheme (PRGS) from the Ministry of Education, Malaysia (Grant No: PR0012020B & PRGS/2/2020/SKK09/UM/02/1)
Publication Title
BMC Infectious Diseases
Volume
22
Issue
1
Publisher
BMC
Publisher Location
CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND