Current trends in polymerase chain reaction based detection of three major human pathogenic vibrios
Document Type
Article
Publication Date
3-9-2022
Abstract
Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus are the most significant aquatic pathogens of the genera Vibrio, account for most Vibrio-associated outbreaks worldwide. Rapid identification of these pathogens is of great importance for disease surveillance, outbreak investigations and food safety maintenance. Traditional culture dependent methods are time-consuming and labor-intensive whereas culture-independent polymerase chain reaction (PCR) based assays are reliable, consistent, rapid and reproducible. This review covers the recent development and applications of PCR based techniques, which have accelerated advances in the analysis of nucleic acids to identify three major pathogenic vibrios. Emphasis has been given to analytical approaches as well as advantages and limits of the available methods. Overall, this review article possesses the substantial merit to be used as a reference guide for the researchers to develop improved PCR based techniques for the differential detection and quantification of Vibrio species.
Keywords
PCR-based detection, Vibrio parahaemolyticus, Vibrio cholera, Vibrio vulnificus, pathogenic, food
Divisions
nanocat
Funders
Universiti Malaya [ST017-2020],University of Malaya [RU001-2020]
Publication Title
Critical Reviews in Food Science and Nutrition
Volume
62
Issue
5
Publisher
Taylor & Francis
Publisher Location
530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA