Surface fabrication of magnetic core-shell silica nanoparticles with perylene diimide as a fluorescent dye for nucleic acid visualization
Document Type
Article
Publication Date
8-1-2022
Abstract
Up to date most traditional fluorescent dyes that serve to detect nucleic acids in a selective manner face growing environmental and economic challenges due to their high toxicity, expensiveness, the long staining times required and the low sensitivities. Further attention went to replace the current widely used ethidium bromide (EB) by more safe and stable dyes but still some of the above drawbacks persist. In this work an innovative fluorescent dye has been designed and synthesized via impregnating perylene diimide into the functionalized surface of magnetic core-shell silica nanoparticles. One of the free hydroxyl groups on the perylene diimide enables the colored magnetic nanoparticles to easily disperse in water and their movement can be controlled with magnetism which allow the separation after their used. These particles are of low toxicity, environmentally friendly and have high sensitivity. Fluorescent properties and molecular interaction were studied. The designed particles show high DNA binding capacity which makes the developed particle promising for DNA extraction, delivery and fluorescent labeling. Theoretical studies confirm that the dye is placed between two DNA base pair similar with pervious observation with EB and the distance was less than 6 A which is much less than the distance of EB dye interaction (15 A) as reported before 1].(c) 2022 Elsevier B.V. All rights reserved.
Keywords
DNA solid-phase extraction, Magnetic nanoparticles, Perylene diimide, Nucleic acid detection, Electrostatic interaction, Magnetic fluorescent dye
Divisions
PHYSICS
Funders
Iraq ministry of higher education,Scientific research and faculty of pharmacy of Al Muthanna university,Department of chemistry of the university of Malaya
Publication Title
Journal of Molecular Liquids
Volume
359
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS