Thermophiles and carbohydrate-active enzymes (CAZymes) in biofilm microbial consortia that decompose lignocellulosic plant litters at high temperatures
Document Type
Article
Publication Date
2-18-2022
Abstract
The SKY hot spring is a unique site filled with a thick layer of plant litter. With the advancement of next-generation sequencing, it is now possible to mine many new biocatalyst sequences. In this study, we aimed to (i) identify the metataxonomic of prokaryotes and eukaryotes in microbial mats using 16S and 18S rRNA markers, (ii) and explore carbohydrate degrading enzymes (CAZymes) that have a high potential for future applications. Green microbial mat, predominantly photosynthetic bacteria, was attached to submerged or floating leaves litter. At the spring head, the sediment mixture consisted of plant debris, predominantly brownish-reddish gelatinous microbial mat, pale tan biofilm, and grey-white filament biofilm. The population in the spring head had a higher percentage of archaea and hyperthermophiles than the green mat. Concurrently, we cataloged nearly 10,000 sequences of CAZymes in both green and brown biofilms using the shotgun metagenomic sequencing approach. These sequences include beta-glucosidase, cellulase, xylanase, alpha-N-arabinofuranosidase, alpha-l-arabinofuranosidase, and other CAZymes. In conclusion, this work elucidated that SKY is a unique hot spring due to its rich lignocellulosic material, often absent in other hot springs. The data collected from this study serves as a repository of new thermostable macromolecules, in particular families of glycoside hydrolases.
Keywords
SP-Nov., Hot-springs, Gen.Nov., Diversity, Discovery, Bacterium
Divisions
Science
Funders
Malaysia Fundamental Research Grant Scheme (FRGS)[5F241],Malaysia Fundamental Research Grant Scheme (FRGS)[5F245]
Publication Title
Scientific Reports
Volume
12
Issue
1
Publisher
Nature Research
Publisher Location
HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY