Correlation of the GC-MS-based metabolite profile of Momordica charantia fruit and its antioxidant activity
Document Type
Article
Publication Date
2-1-2022
Abstract
Momordica charantia or bitter melon (Cucurbitaceae) is a widely consumed edible fruit with strong antioxidant properties. Due to these properties, it has been commercialised by the natural product industries as a coadjutant in the treatment of various ailments attributable to the deleterious effects of oxidants. The present work aimed to evaluate the antioxidant activity of M. charantia fruit extracts made with different compositions of ethanol:water, and to identify the metabolites that are responsible for this activity. To this end, the fruit samples were extracted using six different concentrations of ethanol in water (0, 20, 40, 60, 80, and 100%). Gas chromatography-mass spectrometry (GC-MS) and multivariate data analysis (MVDA) were used to identify significant antioxidants. The 80% ethanol:water extract showed the most significant (p < 0.05) antioxidant activity when tested with the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) antioxidant assays. The multivariate data analysis revealed that the metabolites related to this antioxidant activity were gentiobiose, glucose, galactonic acid, palmitic acid, galactose, mannose, and fructose. (c) All Rights Reserved
Keywords
Antioxidants, DPPH, FRAP, Metabolomics, Momordica charantia
Divisions
InstituteofBiologicalSciences
Funders
International Islamic University Malaysia (Grant No: P-RIGS18-027-0027),Swedish Research Links grant scheme (Grant No: VR 2016-05885),Department of Molecular Biosciences, Wenner-Grens Institute, Stockholm University, Sweden
Publication Title
International Food Research Journal
Volume
29
Issue
1
Publisher
Universiti Putra Malaysia
Publisher Location
SERDANG, SELANGOR, 00000, MALAYSIA