New robustaflavone from Garcinia latissima Miq. leave and Its antibacterial activity
Document Type
Article
Publication Date
1-1-2022
Abstract
Isolation and determination of antibacterial compounds from plants are essential to obtain a new antibacterial as a substitute for conventional resistant antibiotics. This study aims to isolate and identify a new robustaflavone as antibacterial activity from Garcinia latissima Miq. leave. In this study, the isolation process was carried out using column chromatography followed by preparative thin layer chromatography (TLC) based on the TLC profile. The fraction D was tested for anti-bacterial Bacillus subtilis using the TLC bioautography method. The isolates obtained were then identified using 1H-NMR, 13C-NMR, distortionless enhancement by polarization transfer, heteronuclear single quantum coherence, and heteronuclear multiple bond coherence. The Activity assay of the isolate was performed using the microdilution method. A pure compound obtained the result of the separation process with eluent n-hexane: Ethyl acetate (3:2) with R f 0.6. This spot follows the spot in the contact bioautographic result of fraction D, the spot with R f 0.6 gives an inhibition zone. After identifying and purifying the isolate were known as Robustaflavone, this compound has activity against B. subtilis with a (minimum inhibitory concentration) value of 2500 ppm. Robustaflavone successfully isolated and identified from G. latissima leave and its antibacterial activity. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Keywords
Acetic acid ethyl ester, Antibiotic agent, Flavone derivative, Hexane, Robustaflavone, Unclassified drug, Antibacterial activity, Article, Bacillus subtilis, Broth dilution, Carbon nuclear magnetic resonance, Column chromatography, Controlled study, Drug identification, Drug isolation, Garcinia, Garcinia latissima, Heteronuclear multiple bond correlation, Heteronuclear
Divisions
foundation
Funders
Hibah Penelitian Kolaboratif Nasional 2021 Universitas Negeri Jakarta [Grant No: 3/PKM/LPPM/IV/2021]
Publication Title
Journal of Advanced Pharmaceutical Technology and Research
Volume
13
Issue
1
Publisher
Wolters Kluwer Medknow Publications