Optimisation of the extraction method of red christia vespertilionis leaves to yield bioactive phytochemical as monitored by gas chromatography-mass spectrometry; pengoptimuman kaedah pengekstrakan pada daun merah christia vespertilionis untuk menentukan fotokimia bioaktif melalui kromatografi gas-spektrometri jisim
Document Type
Article
Publication Date
1-1-2022
Abstract
Christia vespertilionis (L. f.) Bakh. f. is well-known for treating various contagious diseases. This plant has been recognised among researchers and locals to have anti-inflammatory properties and has thus become popular for treating cancer. Two types of C. vespertilionis are acknowledged which are green and red. The green C. vespertilionis has been widely studied by many researchers, however just a few have studied the red type. This study was carried out to optimise the extraction method of red C. vespertilionis leaves by different extraction techniques (maceration and Soxhlet extraction) and solvents (methanol and ethanol) to yield bioactive phytochemicals using gas chromatography-mass spectrometry (GC-MS). The components were identified through GC-MS via comparisons as guided by the National Institute of Standards and Technology Mass Spectral Library 2011 (NIST 11, version 2.0g). According to the four (4) samples of red C. vespertilionis leaves using maceration of methanol (RMM), maceration of ethanol (RME), Soxhlet extraction of methanol (RSM), and Soxhlet extraction of ethanol (RSE), seventy-one (71) bioactive phytochemicals were identified. Eleven (11) major bioactive phytochemicals (abundance of > 4) were identified namely, acetic acid, butyl ester; 1-butanol, 3-methyl-, acetate; heptanoic acid, propyl ester; hexanoic acid, 3-oxo-, ethyl ester; phenol, 3,5-bis(1,1-dimethylethyl)-; 1-octadecene; 4-O-methylmannose; .alpha.-d-mannofuranoside, methyl; 2-undecene, 9-methyl-, (E)-; n-hexadecanoic acid; and 1-octadecanol. Only seven (7) out of eleven (11) compounds were reported to have biological activities. Among those samples, RSM was the most effective using correlation coefficient between abundance () and retention time (minute(s)) with a significant difference at P < 0.05. © 2022, Malaysian Society of Analytical Sciences. All rights reserved.
Keywords
Christia vespertilionis, gas chromatography-mass spectrometry, maceration, phytochemicals, Soxhlet extraction
Divisions
InstituteofBiologicalSciences
Funders
Universiti Malaya [Grant No: GPF006B-2018]
Publication Title
Malaysian Journal of Analytical Sciences
Volume
26
Issue
2