Integrase deficient lentiviral vector: Prospects for safe clinical applications
Document Type
Article
Publication Date
8-1-2022
Abstract
HIV-1 derived lentiviral vector is an efficient transporter for delivering desired genetic materials into the targeted cells among many viral vectors. Genetic material transduced by lentiviral vector is integrated into the cell genome to introduce new functions, repair defective cell metabolism, and stimulate certain cell functions. Various measures have been administered in different generations of lentiviral vector systems to reduce the vector's replicating capabilities. Despite numerous demonstrations of an excellent safety profile of integrative lentiviral vectors, the precautionary approach has prompted the development of integrase-deficient versions of these vectors. The generation of integrase-deficient lentiviral vectors by abrogating integrase activity in lentiviral vector systems reduces the rate of transgenes integration into host genomes. With this feature, the integrase-deficient lentiviral vector is advantageous for therapeutic implementation and widens its clinical applications. This short review delineates the biology of HIV-1-erived lentiviral vector, generation of integrase-deficient lentiviral vector, recent studies involving integrase-deficient lentiviral vectors, limitations, and prospects for neoteric clinical use.
Keywords
Integrase-deficient lentiviral vector, Gene therapy, Immunization, Cell Reprogramming, Cell death
Divisions
fac_eng
Funders
Ministry of Education, Malaysia [FRGS/1/2020/SKK06/UKM/03/4],Universiti Kebangsaan Malaysia through the Faculty of Medicine grant [FF-2020-327]
Publication Title
PeerJ
Volume
10
Publisher
PeerJ
Publisher Location
341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND