Genetic stability analysis of tissue culture derived date palm cv. Dedhi plants using IRAP markers
Document Type
Article
Publication Date
1-1-2022
Abstract
Phoenix dactylifera cv. Dedhi is an excellent soft date palm cultivar of district Khairpur, Sindh-Pakistan with large size fruit, brittle texture and very low tannins at the Khalal stage. The population of this palm cultivar is currently threatened by pest and disease issues, a limited availability of offshoots and by the prevailing monoculture of semi-dry cultivars together with import of exotic elite cultivars into the region. Hence, the current study aimed to determine genetic stability of large-scale in vitro multiplied materials after their transfer to the field. The palms were phenotyped and then genotyped using two Inter-retrotransposons Amplified Polymorphism (IRAP) markers. Thirteen IRAP primers with 24 combinations were screened, of which 14 combinations produced 301 clear and reproducible bands which were all monomorphic. The IRAP banding patterns showed no variation among the tissue culture derived plants tested. No significant variation in fruit characteristics of the tissue culture derived plants was found when compared to mother plant. Using an optimized micropropagation protocol, number of plants unable to form inflorescences was reduced from 36.7 in the year 2016 to 10 in 2018. The study confirms the current micropropagation protocol to be effective for production of true-to-type plants and the applied marker system to be efficient for validating genetic stability of in vitro produced palms. © 2021 Ecological Society of China
Keywords
Date palm, IRAP, Micropropagation, True-to-type
Divisions
cebar
Funders
Universiti Malaya,Higher Education Commission, Pakistan [Grant no. RU006-2017]
Publication Title
Acta Ecologica Sinica
Volume
42
Issue
1
Publisher
Ecological Society of China