Femtomolar dengue virus type-2 DNA detection in back-gated silicon nanowire field-effect transistor biosensor

Document Type

Article

Publication Date

1-1-2022

Abstract

Dengue is known as the most severe arboviral infection in the world spread by Aedes aegypti. However, conventional and laboratory-based enzyme-linked immunosorbent assays (ELISA) are the current approaches in detecting dengue virus (DENV), requiring skilled and well-trained personnel to operate. Therefore, the ultrasensitive and label-free technique of the Silicon Nanowire (SiNW) biosensor was chosen for rapid detection of DENV. Methods: In this study, a SiNW field-effect transistor (FET) biosensor integrated with a back-gate of the low-doped p-type Silicon-on-insulator (SOI) wafer was fabricated through conventional photo- lithography and Inductively Coupled Plasma - Reactive Ion Etching (ICP-RIE) for Dengue Virus type-2 (DENV-2) DNA detection. The morphological characteristics of back-gated SiNW-FET were examined using a field-emission scanning electron microscope supported by the elemental analysis via energy-dispersive X-ray spectroscopy. Results and Discussion: A complementary (target) single-stranded deoxyribonucleic acid (ssDNA) was recognized when the target DNA was hybridized with the probe DNA attached to SiNW surfaces. Based on the slope of the linear regression curve, the back-gated SiNW-FET biosensor demonstrated the sensitivity of 3.3 nAM(-1 )with a detection limit of 10 fM. Furthermore, the drain and back-gate voltages were also found to influence the SiNW conductance changed. Conclusion: Thus, the results obtained suggest that the back-gated SiNW-FET shows good stability in both biosensing applications and medical diagnosis throughout the conventional photolithography method.

Keywords

Field-effect transistor, Biosensor, Silicon nanowire, back-gate, Conventional photolithography, Dengue virus

Publication Title

Current Nanoscience

Funders

Fundamental Research Grant Scheme (FRGS)[9003-00834 -FRGS/1/2020/STG01/UNIMAP/02/1],Ministry of Education, Malaysia,Universiti Malaysia Perlis (UniMAP

Volume

18

Issue

1

Publisher

Bentham Science Publishers

Publisher Location

EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES

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