Antibacterial activity of salvadora persica and benzylisothiocyanate against prevotella intermedia and eikenella corrodens incorporated into periodontal chips
Document Type
Article
Publication Date
1-1-2022
Abstract
Salvadora persica (S. persica) contains steam-distillable oil made of 90 Benzylisothiocyanate (BITC). It’s proven to have a wide range of bactericidal activity. The objectives of this study were to develop biodegradable periodontal chips containing S. persica, and BITC in a chitosan base and to evaluate their antibacterial activity against Prevotella intermedia and Eikenella corrodens. Periodontal chips were developed that incorporated S. persica (Miswak) extract and BITC in a chitosan base. Sterile distilled water was used as negative control while chlohexidine was used as positive control. A gel diffusion test was performed to detect the potential antibacterial activity followed by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Tests. Scanning electron microscopy (SEM) was used to identify the morphology and configuration of Prevotella intermedia and Eikenella corrodens bacteria before and after treatment with chips. The mean inhibition zone for the S. persica chips was found to be 9.0 mm. The MIC and MBC tests demostrated that all the concentrations for the chips succesfully inhibited the growth of the bacteria. Chlohexidine used as positive control also inhibited the bacteria. The negative control of sterile distilled water showed no inhibtion of bacterial growth. SEM showed that all chips produced distinct alterations to the bacterial morphology. E. corrodens and P. intermedia underwent shrinkage and formed irregular shapes. Periodontal chips formulated from S. persica and BITC incorporated into a chitosan base as a target drug delivery method, showed potent antibacterial activity against E. corrodens and P. intermedia. © 2022. Journal of International Dental and Medical Research. All Rights Reserved.
Keywords
Anti-bacterial activity, Benzyl isothiocyanate chips, Chitosan, Salvadora persica chips
Divisions
fac_med
Funders
University Technology MARA [Grant No: 198/2009, 600-RMI/ST/DANA5/3/ Dst],Hallym University Medical Center
Publication Title
Journal of International Dental and Medical Research
Volume
15
Issue
2
Publisher
University of Dicle