Anticancer activity of 5-Fluorouracil-Loaded Nanoemulsions containing Fe3O4/Au core-shell nanoparticles
Document Type
Article
Publication Date
12-5-2021
Abstract
Nanoemulsions (NEs) as multi-compartmentalized systems are extensively considered as versatile drug delivery systems, due to their multifunctional and tuneable physicochemical properties. Here, water-in oil-in-water (WOW) NEs were formulated using Juglans regia-based Fe3O4/Au-core-shell nanoparticles to encapsulate 5-Fluorouracil (5-FU) for potential colorectal cancer treatment. Response surface methodology, FTIR, TGA, TEM, DLS, VSM, UV-vis spectroscopy, and viscosity measurements were used to characterize the fabricated samples. The analysis confirmed the successful synthesis of Au, Fe3O4, and Fe3O4/Au core-shell nanoparticles with the Juglans regia (J. regia) extract as a green stabilizer and also reducing agent. The NE displayed a spherical-shape and TEM size of similar to 30 nm. It also exhibited good colloidal stability and multifunctional features related to its components. TGA data indicated that 5-FU increased thermal stability after its loading into the nanoemulsion as a drug carrier. 5-FU release from the NE carrier presented a time-delayed behavior and higher release dosage in media at pH 4 compared to pH 7 within 48 h. When tested against colorectal-derived cell lines, 0.78 mu g/mL of 5-FU-loaded Fe3O4/Au core shell NEs showed potent anticancer activity against HCT116 cancerous cells, while exhibiting low toxicity towards CCD112 normal cells. In conclusion, the synthesized 5-FU-loaded NEs can be potently used as an anticancer drug carrier model, owing to its nano-scale size and other functionalities, which should be further studied. (C) 2021 Elsevier B.V. All rights reserved.
Keywords
Nanoemulsion, 5-Fluorouracil, Fe3O4/Au nanoparticles, Core-shell, Colorectal cancer, Juglans regia
Divisions
nanocat
Funders
Takasago Thermal Engineering Co. Ltd. from the research management center (RMC) of Universiti Teknologi Malaysia (UTM) (130000.7343.4B422),Malaysia Japan International Institute of Technology (MJIIT)
Publication Title
Journal of Molecular Structure
Volume
1245
Publisher
Elsevier
Publisher Location
RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS