Electrochemical biosensors with aptamer recognition layer for the diagnosis of pathogenic bacteria: Barriers to commercialization and remediation
Document Type
Article
Publication Date
12-1-2021
Abstract
One of the serious public health threats is pathogenic bacteria due to the lack of affordable and early stage diagnosis and treatment. The existing diagnostic methods are tedious, time-consuming, expensive, and labor-intensive. Therefore, there is an urgent need for achieving simple, low-cost, precise, and rapid detection methods. Besides a plethora of efforts to meet those promises, electrochemical biosensors technologies with aptamers (synthetic ssDNA or ssRNA) recognition moieties have emerged as a promising sensing platform for bacterial diagnosis. In this review, we have comprehensively discussed the recent advances of different potentiometric and non-potentiometric electrochemical aptasensors emphasizing their surface designs, functionalization, and significant performance for the detection of pathogenic bacteria. At the end of this review, various quality advancements of aptasensors technologies, prospects, challenges, and boundaries of commercialization with research directions have been focused on and summarized. (c) 2021 Elsevier B.V. All rights reserved.
Keywords
Aptamers, Nanomaterials, Functionalization, Potentiometric, Non-potentiometric, Aptasensors, Pathogenic bacteria, Commercialization
Divisions
nanocat
Funders
Universiti Malaya [ST030-2019],Universiti Malaya [RU001-2020]
Publication Title
Trac-Trends in Analytical Chemistry
Volume
145
Publisher
Elsevier Science Ltd
Publisher Location
THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND